Abstract: The research about optimation of analytical performance for determination of caffeine by using High Performance Liquid Chromatography (HPLC) method has been done. The optimal of result determination to obtain, the HPLC system has been optimalized for the important parameters are composition of mobile phase, flow rate and eluen pH. The result of research shown that the optimum conditions to obtain from the research are composition of mobile phase methanol: water (70: 30), flow rate 0.5 mL / minute and eluent pH are 5 has been achieved. Based on the optimalized condition, the analytical performance of this method has been performed for the determination of caffeine with HPLC method. The result of research shown reproducibility of this method as coefficient of variation percentage (% CV) was 1.8665 %, limit of detection was 33.1332 ppb. This result showed this method capable to application for the routine analysis of caffeine in samples. Keywords: Caffeine, HPLC, analytical performance
Penulis: Aman Sentosa Panggabean, Subur P. Pasaribu, Nisma Vinanda, Rita Hairani
Kode Jurnal: jpkimiadd100051
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