UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK KASAR ETANOL, FRAKSI n-HEKSANA, ETIL ASETAT DAN METANOL DARI BUAH LABU AIR (Lagenari siceraria (Molina) Standl)

Abstract: Phytochemical test and Antibacterial Activity test in each fraction from the gourds fruit (Lagenari siceraria (Molina) Standl) that came from Teluk Dalam, Tenggarong Seberang have been done. The sample of leaves was extracted with ethanol and then consentrated by using rotary evaporator. The ethanol crude extract were fractioned with n-hexane, Ethyl Acetat and Methanol. Based on the phytochemical test on secondary metabolits of the gourds fruit (Lagenari siceraria (Molina) Standl), it showed that ethanol crude extract contained flavonoid, saponin, steroid and phenol. n-hexane fraction contained steroid, ethyl acetat fraction contained phenol. And methanol-water fraction contained saponin, and phenol. Based on the Antibacterial Activity test using the paper disc diffusion method (diameter 6 mm), the results of this test showed that the most active fraction is n-Heksana with : of the Bacillus cereus bacteria at the concentration 1, 2, 4, 6, 8 and 10% (b/v) with inhibition diameter 9.57, 9.88, 10.17, 12.18, 13 and 14.3 mm respectively so minimum inhibitory concentration got 0-1%. The active respons toward Salmonella typhi bacteria at the concentration 1, 2, 4, 6, 8 and 10% (b/v) with inhibition diameter 7.50, 7.60, 8.20, 8.38, 8.87, and 9.8 mm so minimum inhibitory concentration got 0-1%. The chloramfenikol antibiotics concentration 0.00386 M as a standar to inhibit the growth of Bacillus cereus and Salmonella typhi bacteria with the inhibition diameter 12.58 mm and 15.06 mm respectively.

Keywords: Lagenari siceraria (Molina) Standl, phytochemical, and antibacterial activity

Penulis: Eva Marliana, Chairul Saleh

Kode Jurnal: jpkimiadd100050

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