Abstract: This research was aimed to enhance the protease activity and to determine the partial purification of protease from lactic acid bacteria Pediococcus pentosaceus. Enzyme showed maximum activity at 50o C and pH 7 with casein as a substrate in 10 minutes incubation period. The result showed that the maximum activity of protease was found at 60-75% of ammonium sulphate precipitation. In temperature 50o C, the partial purification degree of protease is 2.135 times higher than crude protease. In pH 7, the partial purification degree of protease is 2.058 times higher than crude protease. In casein, the partial purification degree of protease is 1.797 times higher than crude protease. In 10 minutes incubation period, the partial purification degree of protease is 2.268 times higher than crude protease. Keywords: protease activity, Pediococcus pentosaceus, ammonium sulphate precipitation
Penulis: Wilda Liona Suri, Sumaryati Syukur, Jamsari
Kode Jurnal: jpkimiadd130094
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