Aktivitas Antioksidan Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomea batatas L.) Hasil Budidaya Daerah Saree Aceh Besar

Abstract: This research was aimed to investigate the antioxidant activity of purple sweet potato leaves cultivated  in  Saree,  Aceh  Besar.  The  study  was  conducted  in  two  stages.  The  first  stage included sampel preparation, phytochemical test, λ max determination, testing of antioxidant activity  by  reducing  power  method,  and  TLC  analysis.  In  stage  2  purification  using  column chromatography,  phytochemical  test,  and  antioxidant  activity  test  were  performed.  The sample  was  dissolved  by  using  70%  ethanol  and  1.5  N  HCl  with  a  ratio  of  85:15  (v/v). Phytochemical  test  results  showed  that  the  sample  consisted  of  not  only  flavonoids  but  also tannins.  The  Rf  value  for  both  sample  and  standard  are  respectively  0.55  and  0.61  with absorbance  value  of  0.826  and  0.845.  The  sample  was  then  purified  using  column chromatography  and  25  fractions  were  grouped  based  on  the  color  intensity  abbreviated  as FA,  FB  and  FC.  Photochemical  test  was  then  conducted  to  examine  the  purity  of  obtained fraction and FB was found to be relatively purer than other fractions. Reducing power test of FB  with  variation  of  weight  10  mg,  20  mg,  30  mg,  40  mg,  and  50  mg  gave  the  percentage inhibition  64.1,  6.7,  67.9,  70.3  and  73.6,  respectively,  while  the  percentage  value  of  α- tocopherol  at  simmilar  weight  variation  are  32.1,  36.7,  43.5  and  50.2%.  From  the  result obtained, it  can  be  concluded  that  the  antioxidant  activity  of  ethanol  extract  of  thick  purple sweet potato leaves is higher than α-tocopherol.

Keywords: antioxidant activity, purple sweet potato leaves, reducing power

Penulis: Sulastri, Erlidawati, Syahrial, Muhammad Nazar, Thursina Andayani

Kode Jurnal: jpkimiadd130112

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