DEGRADASI BAHAN ORGANIK LIMBAH CAIR NANAS OLEH BAKTERI INDIGEN

ABSTRACT: Pineapple  Liquid  Waste  has  high  organic  material    and  still  unfulfilled  required  quality standard.  Processing  occurred  at  the  waste  processing  installation  with  pond  system (Lagoon)  required  a  wide  space  and  keep  long  time  so  that  less  efficient.  Based  on  this problem,  it  needs  an  environmentally  sound  waste  management  by  utilizing  bioprocess technology  using  indigenous  bacteria’s  ability  in  degrading organic pollutant  called Bioremediation.  The  objective  of  this  research  was  to  get  isolated  indigenous  bacteria, enables  to  decrease  organic  material.  It  was  divided  into  four  stages.  First,  performed characteristic  observation  of  isolated  bacteria  that  potentially  decrease  organic  material, secondly  decomposition  capability  test  in  vitro,  and  thirdly  scale  bioremediation  test  pilot plan, fourthly bioremediation test in ex situ at the microbiology laboratory of Malang State University.  Data  were  analyzed  by  Varian  analysis  to  test  the  presence  or  absence  of differences and regression test to analyze the link between variables. First phase result: 15 isolated  indigenous  bacteria..  Potential  consortia  bacteria  consisting  of  more  effective  4 species, indicated by 4 consortia bacteria decreased BOD in 6 days. Starter bacteria with a consortia K1’s combination (ABCD), Volume: 5% (v/v) and 6 days were the most effective  incubation  time  in  decreasing  BOD.  Second  phase  result:  1)  A  significant  link among  the  number  of  bacteria,  a.  Bacillus  cereus, b.  Acinetobacter  baumanni,  c. Bacillus  subtilis, d.  Pseudomonas  pseudomallei  and  not  abcd  against  organic  material with contribution of 72,7% and negatively  correlated, 2)  Media  treatment and incubation time  gave  distinctive  outcome  against  organic  material, 3)  there  was  a  link  between organic  material  organic  acid  concentration  decrease  would  decrease  BOD    at  scale bioremediation  test  Pilot  plan,  and  ex  situ  scale.  Threshold  BOD  achievement  limit  value on 10th day (75) quality standard: 75. In accordance with ministry decree LH No. 05 year 2007  industrial  wastewater  quality  standard,  suggested  to  industry  to  use  this  study  in processing its waste.

Keywords: organic material, isolated indigenous bacteria, pineapple liquid waste

Penulis: Agus Sutanto

Kode Jurnal: jpbiologidd110021

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