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GAMBARAN HISTOLOGIS PULMO MENCIT JANTAN (Mus musculus L.) SETELAH DIPAPARI ASAP ROKOK ELEKTRIK

GAMBARAN HISTOLOGIS PULMO MENCIT JANTAN (Mus musculus L.) SETELAH DIPAPARI ASAP ROKOK ELEKTRIK
Abstract: The  effects  of  electric  cigarette  exposure  on  lung  tissue  of  mice  were  studied.  The  experiments  using completely randomized design (CRD) with 3 treatments, i.e.  treatment of electric cigarette with strawberry flavour,  tobacco  flavour,  and  control  treatment  that  was  not  exposed  to  cigarette  smoke.  Eight  male  mice were used for each group. Exposure treatment was done every day for 2 consecutive weeks  with dosage of 20  times  suction  using  syringe  of  60  mL.  After  receiving  treatment  for  2  weeks,  the  mice  were  killed  by cervical  dislocation.  Lung  organ  was  isolated  and  prepared  histologically  using  paraffin  method  and  then stained  with  haematoxylin  and  eosin    (HE).  Result  showed,  treatment  mice  lose  weight  significantly  (p< 0.05). In gross anatomy, electric cigarrete smoke affect lung feature, but histological observation showed that membrane  damage,  damage  to the  lumen  and  the  relationship  between  alveoli  were  statistically unsignificant  (p>0.05).  In  summary,  short  period  exposure  to  electric cigarette  smoke    tends  to  induce  injury  to    lung  at  anatomical  level,  but  in  several  histological  aspects  electric cigarette  treatment has  no significant effect  on lung tissue.
Keywords: alveolar lumen, alveolar membrane, electric cigarettes
Penulis: Nanin Triana, Syafruddin Ilyas dan Salomo Hutahaean
Kode Jurnal: jpbiologidd130006
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