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ISOLASI DAN UJI POTENSI BAKTERI TANAH PERTANIAN BERASTAGI SUMATERA UTARA DALAM MENDEGRADASI FUNGISIDA ANTRACOL BERBAHAN AKTIF PROPINEB

ISOLASI DAN UJI POTENSI BAKTERI TANAH PERTANIAN BERASTAGI SUMATERA UTARA DALAM MENDEGRADASI  FUNGISIDA ANTRACOL BERBAHAN AKTIF PROPINEB
Abstract: Isolation  and  potential  Berastagi  agricultural  soil  bacteria,  North  Sumatra  in  degrading  propineb-based antracol  fungicide  has  been  conducted.  The  isolated  bacteria  were  grown  on  Bushnell  Hass  Broth  (BHB) containing  2%  of  propineb-based  antracol  fungicide  with  propineb  as  the  active  compound.  The  cultures have been grown on shaking incubator at 150 rpm for 21 days. The media BHB containing 2% of propineb-based  antracol  fungicide  without bacteria  was used  as  a  control.  The parameters observed  were  the  growth bacterial,  biosurfactant  activity,  biosurfactant  concentration  and  the  residues  of  propineb  which  were observed on day 0th, 7th, 14th and 21th. A total of sixteen bacterial isolates were isolated using selective media Bushnel  Hass  Agar  (BHA)  containing  2%  propineb-based  antracol  fungicide.  Two  bacterial  isolates  which were CBA 02 and JBA 04 were selected for further test to determine their ability to degrade propineb. JBA 04  showed  much  higher  ability  in  reducing  propineb concentration  up to  60.86%,  while  CBA  02  was  only 5.59% than that of control.
Keywords: biodegradation, bioremediation, biosurfactant, fungicides, propineb
Penulis: Diah Sri Utami, Nunuk Priyani dan Erman Munir
Kode Jurnal: jpbiologidd130004
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