Pengaruh Konsentrasi Substrat Kulit Nanas dan Kecepatan Pengadukan terhadap Pertumbuhan Lactobacillus plantarum untuk Produksi Asam Laktat

Abstract: One of the alternative utilization of pineapple peel waste is to convert them to lactate acid via fermentation. This research is aimed to obtain better understanding on the growth of lactate bacteria (Lactobacillus plantarum)  by varying substrate concentration:  100 g/L  - 600  g/L  and  stirring  rate  of  50  rpm,  150  rpm,  and  250  rpm.  The  growth  of  anaeroic process was performed without pH controlling with initial pH of 5 and temperature of 28^OC.  Pineapple peel was used as the substrate after romoving water content using ammonium dihydrogen phosphate.  Cell growth was analyzed by calculating the total of microorganism cell  using  hemocytometer.  In  order  to  determine  lactate  acid  production,  glucose concentration  was  analyzed  using  spectrophotometer.  Nelson-Somogy  method  was employed  in  this  analysis.  Ethanol  concentration  was  also  analyzed  by  calculating  the titration of acid lactate concentration. The optimum growth of Lactobacillus plantarum was found at 28oC, stirring rate of 150 rpm and substrate concentration of 500 g/L contributing to the total cell  of  3,85.108, 1,13.109, and  4,00.108  for lag, exponential, and decrease phase  respectively.    Further  analysis  carried  out  based  on  stirring  rate  of  150  rpm  and substrate concentration of 500 g/L resulted in the largest average concentration of lactate acid of 1.620% v/v with glucose consumption percentage of 51,76%. 

Keywords: pineapple peel, Lactobacillus plantarum, lactate acid

Penulis: Panca Nugrahini Febriningrum

Kode Jurnal: jpkimiadd130109

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