PENGARUH PENAMBAHAN TEMBAGA SULFAT TERHADAP PRODUKSI ENZIM POLIFENOL OKSIDASE DARI FUNGI ENDOFIT KULIT BUAH KAKAO

ABSTRACT: In  medicine,  polyphenol  oxidases  are used  for prevention  of  bacterial  adhesion,  treatment of Parkinson’s disease and control of melanin synthesis. Polyphenol oxidase could be produce by endophytic fungus from podhusk of Theobroma cacao. To produce microbial enzyme, media composition should be optimized. The aim of this study was to investigate the effect of cupri sulphate concentration as inducer  in  fermentation medium containing 1 % yeast extract, 0,05 % MgSO₄, 0.1% KH₂PO₄, 0,051 % gallic acid, and CuSO₄.5H₂O  0-50  ppm.  The  result  showed  that it  was  effect  addition of CuSO₄in fermentation medium toward production of polyphenol oxidase. Optimal concentration of CuSO₄ to produce polyphenol oxidase was 25 bpj with enzyme activity  530 U/mL using catechol as substrate.

Keywords:endophytic fungus, cocoa podhusk, cupri sulphate, polyphenol oxidase                 

Penulis: Sartini , Abd.Rauf Patong, Tjodi Harlim, dan Pirman

Kode Jurnal: jpfarmasidd110006

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