BIOAKTIVITAS ANTIBAKTERI FRAKSI PROTEIN ALGA MERAH Gelidium amansii DARI PERAIRAN CIKOANG KABUPATEN TAKALAR, SULAWESI SELATAN

ABSTRACT: A research on the ability of protein fraction from  red algae Gelidium amansii to inhibit the growth of  Salmonella thypi and Staphylococcus aureus has been conducted. Proteins was fractionated from the crude extract by salting out method with  0 – 20 %, 20 – 40 %, 40 – 60 % and 60 – 80 % ammonium sulfat saturation. Proteins was purified by a dialysis method using a selophan membrane. The protein level was determined by Lowry’s method, the highest protein concentration, 5.6 mg/mL was found in the 40 – 60 % fraction  of the red algae. Antibacterial activity test was performed using a layered jelly diffusion method on the MHA  medium.  Results  indicated  that  crude  extract  and  the  protein  fraction  of  the  red  algae  had antibacterial  effect  to  Salmonella  thypi  and  Staphylococcus  aureus.  The  highest  antibacterial  activity  to Staphylococcus  aureus  was  found  in  the  0-20%  fraction  with  the  inhibition  diameter  of  14.43  mm  after incubated for 24 hours. These research might provide a significant results for the following research on the antibacterial action in molecular  level of protein compounds from marine algae. 

Keywords: antibacterial, Lowry’s method, protein fraction, red algae

Penulis: Seniwati Dali, Hasnah Natsir, Hanapi Usman dan Ahyar Ahmad

Kode Jurnal: jpfarmasidd110015

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